Nanosensor Design for Bio-detection - overview
Nano-sensors that provide real-time monitoring of bioanalytes (small molecules, micro-RNAs, proteins) have a wide range of applications from health monitoring to diagonistics to disease prevention. Design of an automated, label-free, highly miniaturized sensor platform is therefore highly crucial for accurate measurement. Toward this goal, in collaboration with Sufi Zafar (IBM), we are providing optimal sensor design protocol by combining device fabrication, electrical measurements, and computational modeling. Recently, we have studied the real-time hybridization of a surface-attached DNA sequence. Our experimental-modeling approach shows presence of conformational heterogeneities corresponding to partially hybridized structures on the surface, which results in false positives and false negatives during sequence detection. We propose a solution by customizing the sensor surface area according to the molecular dimension, which will increase the diagnostic accuracy of hybridization-based DNA sequence detection methods.
1. Das, Payel, and Sufi Zafar. "Mechanistic Influence of Nanometer Length-Scale Surface Chemistry on DNA Hybridization." ACS nano 9.7 (2015): 7466-7478.